摘要
We investigated the kinetics of Hg(II) and MeHg accumulation and the synthesis of phytochelatins (PCs), cysteine (Cys), glutathione (GSH), and 纬-glutamylcysteine (纬-EC) in a marine diatom Thalassiosira weissflogii during a 3-h (short-term) and a 96-h (long-term) exposure period, and during a subsequent 96-h recovery period. MeHg induced the synthesis of a significant level of GSH, but it was Hg(II) that gave rise to significant levels of other non-protein thiol compounds. The thiol compounds Cys, 纬-EC, and PC2-3 were induced in T. weissflogii within the first 30 min of exposure, followed by PC4, but the concentrations of all six compounds returned to the control levels after the 96-h recovery period. The kinetics of these non-protein thiol compounds pointed to a rapid cellular response to environmental mercury pollution. After a first decrease, the molar ratio of PC-SH (sulfhydryl in PCs) to intracellular Hg increased slightly which demonstrated the role of PCs in Hg(II) detoxification. However, PC-SH was bound with Hg(II) at a stoichiometric ratio of 0.1-0.3, indicating the involvement of other detoxification mechanisms. Elucidating the effects of mercury on intracellular non-protein thiol pools may help us better understand the metal detoxification in phytoplankton.