Quantitative and qualitative characterization of plasma DNA identifies primary and recurrent colorectal cancer

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• 作者：M. Frattini ; G. Gallino ; S. Signoroni ; D. Balestra ; L. Lusa ; L. Battaglia ; G. Sozzi ; L. Bertario ; E. Leo ; S. Pilotti ; M.A. Pierotti
• 关键词：Plasma ; Quantitative analysis ; Colorectal cancer ; K-Ras ; p16^INK4a
• 刊名：Cancer Letters
• 出版年：2008
• 期刊代码：44_03043835
• 类别：med
• 出版时间：18 May 2008
• 卷：263
• 期：2
• 页码：170-181
• 文件大小：662 K

摘要

Because plasma DNA may be a useful tool for cancer detection, we screened primary tumors and related multiple plasma samples at the time of surgery and during the follow-up period for plasma DNA level as well as for K-Ras mutations and p16^INK4a promoter hypermethylation in colorectal cancer patients. At the time of surgery, DNA levels were higher in tumor patients than in healthy donors, and K-Ras and p16^INK4a alterations were detected in 7 and 11 cancers respectively, and in all related plasma samples. During the follow-up, plasma DNA levels decrease progressively but rapidly increased when a relapse occurred, whereas K-Ras and p16^INK4a alterations were detected only in relapsed patients. Therefore, combined quantitative and qualitative analyses of plasma DNA confirm the presence of colorectal cancer, define disease-free status and indicate the presence of relapse.