Cloning, characterization, and expression analysis of a novel BmGDAP1 gene from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection

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• 作者：Kun Gao^a ; ^b ; ^c ; Xiangyuan Deng^c ; Heying Qian^a ; ^b ; Ping Wu^a ; ^b ; Guangxing Qin^a ; ^b ; Xijie Guo^a ; ^b ; ^{guoxijie@126.com}
• 关键词：BmGDAP1 ; Ganglioside-induced differentiation-associated protein 1 gene from Bombyx mori ; RACE ; rapid amplification of cDNA ends ; ORF ; open reading frame ; BmCPV ; Bombyx mori cytoplasmic polyhedrosis virus ; TRX ; thioredoxin ; GST-C ; glutathione S-transferase
• 刊名：Gene
• 出版年：2012
• 期刊代码：73_03781119
• 类别：bio
• 出版时间：15 April, 2012
• 卷：497
• 期：2
• 页码：208-213
• 文件大小：395 K

摘要

A novel ganglioside-induced differentiation-associated protein 1 gene (BmGDAP1) was first cloned and sequenced from silkworm, Bombyx mori using rapid amplification of cDNA ends (RACE). The full-length cDNA of BmGDAP1 was 1514 bp, consisting of a 91 bp 5鈥?untranslated region (UTR), a 424 bp 3鈥?UTR and a 999 bp open reading frame (ORF). The ORF encoded a polypeptide of 332 amino acids, which possessed a thioredoxin (TRX)-like domain, a glutathione S-transferase-C (GST-C) family domain and a transmembrane segment. Furthermore, quantitative real-time PCR analysis revealed that BmGDAP1 transcripts were mainly presented in the tissues of hemocytes and midgut of silkworm, and its expression level was down-regulated in the hemocytes, while up-regulated in the midgut. Therefore, it could be concluded that BmGDAP1 plays an important role in the recognition and immune response of silkworm to BmCPV infection.