17尾-Estradiol stimulates the translocation of endogenous estrogen receptor 伪 at the plasma membrane of normal anterior pituitary cells

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• 作者：Silvina Gutié ; rrez^a ; ^{silvina@cmefcm.uncor.edu} ; Liliana d V. Sosa^a ; ² ; Juan P. Petiti^a ; ² ; Jorge H. Mukdsi^a ; ² ; Ivá ; n D. Mascanfroni^b ; ¹ ; ³ ; Claudia G. Pellizas^b ; ³ ; Ana L. De Paul^a ; ² ; Marí ; a J. Cambiasso^c ; ⁴ ; Alicia I. Torres^a ; ²
• 关键词：ER ; estrogen receptor ; PPT ; 4 ; 4&prime ; 4&Prime ; -(4-propyl-[1H]-pyrazole-1 ; 3 ; 5-tryl) trisphenol ; selective ER伪 agonist ; ICI 182780 ; (7a ; 17b)-7-[9-[(4 ; 4 ; 5 ; 5 ; 5pentafluoropentyl) sulfinyl]nonyl]estra-1 ; 3 ; 5(10)-triene-3 ; 17-diol ; PKC ; protein kinase C
• 刊名：Molecular and Cellular Endocrinology
• 出版年：2012
• 期刊代码：109_03037207
• 类别：bio
• 出版时间：15 May, 2012
• 卷：355
• 期：1
• 页码：169-179
• 文件大小：1642 K

摘要

In the present work we aimed at identifying ER伪 in the plasma membrane of normal anterior pituitary cells and investigated if 17尾-estradiol was able to induce their subcellular redistribution. Our results show that about 8%of anterior pituitary cells expressed ER伪 in the plasma membrane, with the geometrical mean fluorescence intensity being increased after steroid hormone treatment. 17尾-Estradiol and the selective ER伪 agonist PPT induced an increase of ER伪 expression in the plasma membrane and activated the PKC伪/ERK 1/2 pathway in a time-course not compatible with genomic actions, thus supporting the notion of membrane-initiated effects. These findings suggest that 17尾-estradiol stimulates the translocation of endogenous ER伪 to the plasma membrane, consequently modulating this ER pool and leading to cellular biological effects in normal anterior pituitary gland.