Identification of new biomarkers for Down's syndrome in maternal plasma

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• 作者：Wendy Heywood^a ; Kevin Mills^a ; Darrell Wang^a ; Julie Hogg^b ; Tracey E. Madgett^c ; Neil D. Avent^c ; Lyn S.Chitty^a ; ^b ; ^{l.chitty@ich.ucl.ac.uk}
• 关键词：DS ; Down's syndrome ; SAMP ; Serum Amyloid P ; C1-inhibitor ; Plasma C1-protease inhibitor ; SELDI TOF MS ; surface-enhanced laser desorption ionisation time of flight mass spectrometry ; NSC ; National Screening Committee ; PLGS ; ProteinLynx Global Server ; ffDNA
• 刊名：Journal of Proteomics
• 出版年：2012
• 期刊代码：P37_18743919
• 类别：ch
• 出版时间：17 May, 2012
• 卷：75
• 期：9
• 页码：2621-2628
• 文件大小：732 K

摘要

Using ProteinChip Technology (SELDI TOF MS), the maternal plasma of 53 chromosomally-normal control and 28 Down's syndrome affected pregnancies was profiled between 10 and 20 weeks' gestation. Preliminary studies demonstrated two distinct phases of changes in protein expression, the first at 10-14 weeks and second at 14-20 weeks. Using this data, analysis of the 10-14 weeks' plasma samples (Down's syndrome n = 13, control n = 20) showed the presence of a protein of mass 100.3 kDa that was elevated in the Down's syndrome group compared to the controls (p < 0.002). This protein was further isolated using SAX Q-spin columns and identified using QTOF MS and Western blotting as being plasma protease C1-inhibitor. Analysis of the 14-20 week cohort demonstrated changes in protein expression of three additional proteins. Two of these proteins were found to be up-regulated (serum amyloid P-component, p < 0.004 and transthyretin, p < 0.006) and complement C3-伪 chain was observed to be down-regulated (p < 0.0005). The identification of these biomarkers in maternal plasma and their potential to improve current Down's syndrome screening are discussed.