Comparison of the immunological and protective responses elicited by microencapsulated formulations of the F1 antigen from Yersinia pestis

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• 作者：Reddin ; Karen M. ; Easterbrook ; Timothy J. ; Eley ; Stephen M. ; Russell ; Paul ; Mobsby ; Vivien A. ; et. al.
• 关键词：Yersinia pestis ; F1 antigen ; antigen delivery ; vaccination ; PLG microspheres ; liposome
• 刊名：Vaccine
• 出版年：1998
• 期刊代码：89_0264410x
• 类别：imm
• 出版时间：May, 1998
• 卷：16
• 期：8
• 页码：761-767
• 文件大小：540 K

摘要

Purified native F1 antigen from Yersinia pestis was used to assess controlled-release vaccine delivery systems in poly(lactide-co-glycolide) (PLG) microparticles and liposomes. Antigen encapsulated in PLG microparticles induced high serum titres when injected i.p. in mice: mucosal IgA was also detected. Mice immunized with F1 in Alhydrogel or PLGs were protected against subcutaneous challenge with Y. pestis. F1 antigen surface-labelled onto liposome vesicles stimulated high serum titres in Balb/c mice and also induced a mucosal response: F1-labelled liposomes protected mice against challenge with up to 1 × 10⁵ organisms. These findings indicate that a significant immune response is induced by immunizing with F1 formulated in PLGs and liposomes and that protection was achieved after only one dose.