JNP3, a new compound, suppresses PMA-induced tumor cell invasion via NF-魏B down regulation in MCF-7 breast cancer cells

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• 作者：Hai Yang Yu¹ ; Kyoung-Sook Kim¹ ; Hyung-In Moon¹ ; Kyung-Mi Kim ; Young-Choon Lee ; Jai-Heon Lee ; ^{jhnlee@dau.ac.kr}
• 关键词：JNP3 ; MMP-9 ; MCF-7 cells ; Invasion ; NF-魏B
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：4 May, 2012
• 卷：421
• 期：2
• 页码：190-196
• 文件大小：898 K

摘要

The expression of matrix metalloproteinase (MMPs)-9 is critical for cell migration and can lead to invasion and metastasis of cancer cells. In the present study, we examined the inhibitory effects of JNP3, a new compound which was isolated from traditional Chinese medicine, on cell invasion and MMP-9 activation in phorbol myristate acetate (PMA)-induced MCF-7 cells. Treatment with JNP3 significantly and selectively inhibited PMA-induced MMP-9 secretion, mRNA expression and protein levels, and these results led to reduction of cell invasion and migration in PMA-induced MCF-7 cells. The results of MMP-9 promoter assay and EMSA showed that JNP3 specifically inhibited PMA-induced MMP-9 gene expression by blocking NF-魏B-dependent transcriptional activity. In addition, PMA-induced phosphorylation of ERK1/2 and JNK were suppressed by JNP3 treatment, whereas the phosphorylation of p38 MAPK was not affected by JNP3. These results suggest that JNP3 can be potential anti-cancer agents through specific inhibition of NF-魏B-dependent MMP-9 gene expression.