Messenger RNA expression of glomerular podocyte markers in the urinary sediment of acquired proteinuric diseases

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• 作者：Cheuk-Chun Szeto ; Ka-Bik Lai ; Kai-Ming Chow ; Carol Yi-Ki Szeto ; Thomas Wai-Cheong Yip ; Kam-Sang Woo ; Philip Kam-Tao Li and Fernand Mac-Moune Lai
• 刊名：Clinica Chimica Acta
• 出版年：2005
• 期刊代码：54_00098981
• 类别：med
• 出版时间：2005
• 卷：361
• 期：1-2
• 页码：182-190
• 文件大小：134 K

摘要

Background

Podocyte slit diaphragm plays an important role in the control of glomerular permeability. We hypothesize that studying the gene expression profile of podocyte in urinary sediment may provide diagnostic and prognostic information on acquired proteinuric diseases.

Methods

We studied 28 patients who required kidney biopsy for acquired proteinuric diseases (diabetic glomerulosclerosis, 9 cases; IgA nephropathy, 10 cases; minimal change disease, 5 cases; membranous nephropathy, 5 cases). We also studied 10 cases of diabetic microalbuminuria and 9 healthy controls. The mRNA expressions of nephrin (NephRNA), podocin (PodRNA) and synaptopodin (SynRNA) in urinary sediment were measured by real time quantitative PCR. After recruitment, all patients were followed for at least 12 months.

Results

There were significant differences in the NephRNA and PodRNA in the urinary sediment between diagnosis groups (p < 0.005). On the other hand, SynRNA was only marginally significant between diagnosis groups (p < 0.05). Although statistically significant, the degree of proteinuria had only modest correlations with the urinary expression of nephrin. After a median follow up for 23 months, there was a significant correlation between the rate of decline in renal function and NephRNA (r = 0.559, p = 0.001) and PodRNA (r = 0.530, p = 0.002), but not SynRNA (r = 0.054, p = NS). The correlation remained statistically significant after multivariate analysis to adjust for the degree of proteinuria and initial renal function.

Conclusions

Urinary mRNA expression of podocyte markers, such as nephrin and podocin, are significantly different between proteinuric disease categories. Further, NephRNA and PodRNA correlated with the rate of decline in renal function. Our results suggest that urinary podocyte gene expression may be a useful non-invasive tool which provides additional information for the management of proteinuric diseases.