Alterations in differentially expressed genes by exposure to a mixture of carcinogenic polycyclic aromatic hydrocarbons in the liver of Oryzias latipes

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• 作者：Jeong Hwan Oh^a ; Hyo-Bang Moon^b ; Eun Sang Choe^a ; ^{eschoe@pusan.ac.kr}
• 关键词：ACP ; annealing control primer ; BaA ; benzo[a]anthracene ; BaP ; benzo[a]pyrene ; BbF ; benzo[b]fluoranthene ; BkF ; benzo[k]fluoranthene ; cPAHs ; carcinogenic polycyclic aromatic hydrocarbons ; CYP450 2P1 ; cytochrome P450 2P1 ; DBA ; dibenzo[a ; h]anthracene ; DEGs ; di
• 刊名：Environmental Toxicology and Pharmacology
• 出版年：2012
• 期刊代码：13_13826689
• 类别：pha
• 出版时间：May, 2012
• 卷：33
• 期：3
• 页码：403-407
• 文件大小：481 K

摘要

The effects of a mixture of carcinogenic polycyclic aromatic hydrocarbons (cPAHs) on transcriptional responses in the liver of medaka, Oryzias latipes, were investigated by identifying differentially expressed genes (DEGs). Five DEGs were identified as cytochrome P450 2P1 (CYP450 2P1), malate dehydrogenase, anti-thrombin III, NADH dehydrogenase subunit 4, and transferrin. These DEGs were quantified by real-time polymerase chain reaction. Only CYP450 2P1 mRNA was found to be upregulated by exposure to cPAHs mixture, suggesting that CYP450 2P1 mRNA can be a potential marker for prediction of the biological effects of a mixture of cPAHs on fish.