Tunable thick polymer coatings for on-chip electrophoretic protein and peptide separation

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• 作者：Mei He^a ; Yong Zeng^a ; Abebaw B. Jemere^b ; D. Jed Harrison^a ; ^b ; ^{jed.harrison@ualberta.ca}
• 关键词：Microfluidics ; Thick polymer coating ; Capillary electrophoresis ; Open-tubular electrochromatography
• 刊名：Journal of Chromatography A
• 出版年：2012
• 期刊代码：47_00219673
• 类别：ch
• 出版时间：8 June, 2012
• 卷：1241
• 期：Complete
• 页码：112-116
• 文件大小：664 K

摘要

We report a variety of procedures for fabricating confinement-induced polymer coatings, used to eliminate non-specific protein adsorption and to control electroosmotic flow for microchip capillary electrophoresis. The coating strategy generates relatively thick polymer wall coatings (100-700 nm) and can easily be tuned by adjusting the monomer concentration. 2-hydroxyethyl methacrylate (HEMA) polymer coating, photopatterned in microfluidic channels, effectively reduced protein non-specific adsorption and rendered high efficiency (N/m = 鈭? 脳 10⁶) for protein separation. The coating strategy provides rapid and effective means to create robust wall coatings, with the ability to photograft various surface chemistries onto the coating. [2-(methacryloyloxy) ethyl] trimethylammonium chloride grafted HEMA coated channels showed high durability and reproducibility for generating EOF (RSD = 2.6%, n = 64) over a period of 15 days. Sulfobetaine methacrylate grafted HEMA coated channels allowed separation of BSA digest, 15 peaks resolved in 25 s, with an average N/m of 4 脳 10⁵.