A poly(l-lysine)-based hydrophilic star block co-polymer as a protein nanocarrier with facile encapsulation and pH-responsive release

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• 作者：Yunsong Yan^a ; Daixu Wei^b ; ^{daviddxwei@163.com} ; Jiayan Li^a ; Jinhong Zheng^a ; Ganggang Shi^a ; Wenhong Luo^a ; Ying Pan^a ; Jinzhi Wang^a ; Lumian Zhang^a ; Xiaoying He^a ; Daojun Liu^a ; ^{liudj@stu.edu.cn}
• 关键词：Star block co-polymer ; Drug delivery system ; Poly(l-lysine) ; Insulin ; Protein
• 刊名：Acta Biomaterialia
• 出版年：2012
• 期刊代码：1_17427061
• 类别：ce
• 出版时间：July, 2012
• 卷：8
• 期：6
• 页码：2113-2120
• 文件大小：743 K

摘要

A hydrophilic star block co-polymer was synthesized, characterized, and evaluated as a protein nanocarrier. The star block co-polymer was composed of a hyperbranched polyethylenimine (PEI) core, a poly(l-lysine) (PLL) inner shell, and a poly(ethylene glycol) (PEG) outer shell. The model protein insulin can be rapidly and efficiently encapsulated by the synthesized polymer in aqueous phosphate buffer at physiological pH. Complexation between PEI-PLL-b-PEG and insulin was investigated using native polyacrylamide gel electrophoresis. The uptake of enhanced green fluorescent protein into Ad293 cells mediated by PEI-PLL-b-PEG was also investigated. The encapsulated insulin demonstrated sustained release at physiological pH and showed accelerated release when the pH was decreased. The insulin released from the star block co-polymer retained its chemical integrity and immunogenicity.