The HLA-G 14bp gene polymorphism and decidual HLA-G 14bp gene expression in pre-eclamptic and normal pregnancies
- 作者：Ann-Charlotte Iversen ; Olav Toai Duc Nguyen ; Linda Fø ; ll Tø ; mmerdal ; Irina Poliakova Eide ; Veslemø ; y Malm L ; sem ; Nuray Acar ; Ronny Myhre ; Helge Klungl ; Rigmor Austgulen
- 关键词：Decidua ; Gene polymorphism ; HLA-G ; Pre-eclampsia ; Pregnancy
- 刊名：Journal of Reproductive Immunology
- 出版年：2008
- 期刊代码：171_01650378
- 类别：med
- 出版时间：July 2008
- 卷：78
- 期：2
- 页码：158-165
- 文件大小：244 K
摘要
Trophoblast expression of the non-classical MHC, HLA-G, is considered essential for feto-maternal immune tolerance and successful placentation in pregnancy. The HLA-G 14 bp polymorphism in the 3′-untranslated region (UTR) of the HLA-G gene has been reported to be associated with development of pre-eclampsia (PE). In this study, maternal (peripheral blood, n = 54) and fetal (cord blood, n = 57) HLA-G 14 bp genotypes have been determined by PCR in pre-eclamptic and normal pregnancies. In addition, HLA-G 14 bp gene expression in decidua basalis (n = 59) was analyzed by RT-PCR. The pre-eclamptic syndrome was neither associated with the HLA-G 14 bp genotype (maternal or fetal), nor with altered decidual HLA-G 14 bp gene expression. Furthermore, the HLA-G 14 bp mRNA expressed in decidua basalis was of fetal origin and all potential transcripts, as predicted from the fetal HLA-G 14 bp genotype, were expressed. In contrast to previous findings, we found no correlation between the HLA-G 14 bp polymorphism and fetal growth. In conclusion, the fetal HLA-G 14 bp genotype is reflected in the decidual HLA-G mRNA splice form profile, but does not appear to be associated with increased risk for development of PE.