Predominance of pHK01-like incompatibility group FII plasmids encoding CTX-M-14 among extended-spectrum beta-lactamase-producing Escherichia coli in Hong Kong, 1996-2008

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• 作者：Pak Leung Ho^a ; ^b ; ^{plho@hkucc.hku.hk} ; Man Kiu Yeung^a ; Wai U Lo^a ; Herman Tse^a ; Zhen Li^a ; Eileen L. Lai^a ; Kin Hung Chow^a ; Kelvin K. To^a ; Wing Cheong Yam^a
• 关键词：Enterobacteriaceae ; CTX-M-14 beta-lactamase ; Antimicrobial drug resistance ; Plasmids ; Restriction fragment length polymorphism
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2012
• 期刊代码：79_07328893
• 类别：med
• 出版时间：June, 2012
• 卷：73
• 期：2
• 页码：182-186
• 文件大小：185 K

摘要

This study assessed the temporal changes in the molecular epidemiology of bacteremic Escherichia coli isolates producing CTX-M-14 in Hong Kong. Blood isolates from 1996 to 1998 (period 1, n = 50) and 2007 to 2008 (period 2, n = 117) were investigated by molecular methods. CTX-M-type ESBL was carried by 98.2%(164/167) of the isolates. In both periods, the CTX-M-9 group and CTX-M-14 allele were the predominant ESBL type. The major clones were found to change from ST68 and ST405 in period 1 to ST131, ST69, and ST12 in period 2. Among 65 CTX-M-14-producing plasmids investigated further, 54 had the FII replicon. Replicon sequence typing and plasmid polymerase chain reaction-restriction fragment length polymorphism showed that 79.6%(43/54) of the FII plasmid subset was similar to the completely sequenced plasmid, pHK01 (human urine, Hong Kong, 2004). These pHK01-like plasmids were found to have spread to the major clones (ST68, ST405, and ST131) and multiple singleton isolates of all 4 phylogenetic groups.