Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering

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• 作者：Yuji Yamada^a ; Kentaro Hozumi^a ; Akihiro Aso^b ; Atsushi Hotta^b ; Kazunori Toma^c ; Fumihiko Katagiri^a ; Yamato Kikkawa^a ; Motoyoshi Nomizu^a ; ^{nomizu@toyaku.ac.jp}
• 关键词：ECM (extracellular matrix) ; Laminin ; Peptide ; Polysaccharide ; Cell culture
• 刊名：Biomaterials
• 出版年：2012
• 期刊代码：7_01429612
• 类别：ms
• 出版时间：June, 2012
• 卷：33
• 期：16
• 页码：4118-4125
• 文件大小：1181 K

摘要

Cell adhesive peptides derived from extracellular matrix components are potential candidates to afford bio-adhesiveness to cell culture scaffolds for tissue engineering. Previously, we covalently conjugated bioactive laminin peptides to polysaccharides, such as chitosan and alginate, and demonstrated their advantages as biomaterials. Here, we prepared functional polysaccharide matrices by mixing laminin active peptides and agarose gel.聽Several laminin peptide/agarose matrices showed cell attachment activity. In particular, peptide AG73 (RKRLQVQLSIRT)/agarose matrices promoted strong cell attachment and the cell behavior depended on the stiffness of agarose matrices. Fibroblasts formed spheroid structures on the soft AG73/agarose matrices while the cells formed a monolayer with elongated morphologies on the stiff matrices.聽On the stiff AG73/agarose matrices, neuronal cells extended neuritic processes and endothelial cells formed capillary-like networks. In addition, salivary gland cells formed acini-like structures on the soft matrices. These results suggest that the peptide/agarose matrices are useful for both two- and three-dimensional cell culture systems as a multifunctional biomaterial for tissue engineering.