Maternal prenatal omega-3 fatty acid supplementation attenuates hyperoxia-induced apoptosis in the developing rat brain

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• 作者：Funda Tuzun^a ; Abdullah Kumral^a ; Seda Ozbal^b ; Mustafa Dilek^a ; Kaz谋m Tugyan^b ; Nuray Duman^a ; Hasan Ozkan^a ; ^{hozkandeu@gmail.com}
• 关键词：蠅-3 FA ; omega-3 fatty acid ; DHA ; docosahexaenoic acid ; EPA ; eicosapentaenoic acid ; TUNEL ; terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling ; NPD1 ; neuroprotectin D1 ; AMPA ; 2-aminomethyl phenyl acetic acid
• 刊名：International Journal of Developmental Neuroscience
• 出版年：2012
• 期刊代码：28_07365748
• 类别：neu
• 出版时间：June, 2012
• 卷：30
• 期：4
• 页码：315-323
• 文件大小：2727 K

摘要

Supraphysiologic amounts of oxygen negatively influences brain maturation and development. The aim of the present study was to evaluate whether maternal 蠅-3 long-chain polyunsaturated fatty acid (蠅-3 FA) supplementation during pregnancy protects the developing brain against hyperoxic injury. Thirty-six rat pups from six different dams were divided into six groups according to the diet modifications and hyperoxia exposure. The groups were: a control group (standard diet + room air), a hyperoxia group (standard diet + 80%O₂ exposure), a hyperoxia + high-dose 蠅-3 FA-supplemented group, a hyperoxia + low-dose 蠅-3 FA-supplemented group, a room air + low-dose 蠅-3 FA-supplemented + group, and a room air + high dose 蠅-3 FA-supplemented group. The 蠅-3 FA's were supplemented as a mixture of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) from the second day of pregnancy until birth. Rat pups in the hyperoxic groups were exposed to 80%oxygen from birth until postnatal day 5 (P5). At P5, all animals were sacrificed. Neuronal cell death and apoptosis were evaluated by cell count, TUNEL, and active Caspase-3 immunohistochemistry. Histopathological examination showed that maternally 蠅-3 FA deficient diet and postnatal hyperoxia exposure were associated with significantly lower neuronal counts and significantly higher apoptotic cell death in the selected brain regions. 惟-3 FA treatment significantly diminished apoptosis, in the selected brain regions, in a dose dependent manner. Our results suggest that the maternal 蠅-3 FA supply may protect the developing brain against hyperoxic injury.