Improved biocompatibility of poly(vinylpyrrolidone)-graft-poly(2-dimethylaminoethyl methacrylate)/DNA complexes by coating with bovine serum albumin

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• 作者：Biao Zhang^a ; Lian Dong Deng^a ; Jin Feng Xing^a ; ^{jinfengxing@tju.edu.cn} ; Jun Yang^b ; An Jie Dong^a
• 关键词：Copolymer ; Electrostatic interaction ; Bovine serum albumin ; Gene delivery
• 刊名：Chinese Chemical Letters
• 出版年：2012
• 期刊代码：3_10018417
• 类别：ch
• 出版时间：May, 2012
• 卷：23
• 期：5
• 页码：627-630
• 文件大小：510 K

摘要

Bovine serum albumin (BSA) was utilized to assemble with the binary complexes of poly(vinylpyrrolidone)-graft-poly(2-dimethylaminoethyl methacrylate) (PVP-g-PDMAEMA)/DNA formed by layer-by-layer electrostatic interactions to screen the residual surface positive charges of complexes. The coating of BSA was able to decrease the zeta potential of binary complexes nearly to electroneutrality without interfering with DNA condensation ability. The ternary complexes of BSA/PVP-g-PDMAEMA/DNA demonstrated lower cytotoxicity compared with the binary complexes and also maintained high gene transfection efficiency in HepG2 cells.