Assessment of population genetic diversity of Fagopyrum tataricum using SSR molecular marker

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• 作者：Garima Kishore^a ; ^{garimadixit2007@gmail.com} ; Sanjay Gupta^b ; Anjana Pandey^a
• 关键词：Genetic diversity ; SSR ; Population differentiation ; Fagopyrum tataricum
• 刊名：Biochemical Systematics and Ecology
• 出版年：2012
• 期刊代码：21_03051978
• 类别：abs
• 出版时间：August, 2012
• 卷：43
• 期：Complete
• 页码：32-41
• 文件大小：302 K

摘要

In this work, the analysis of genetic diversity of Fagopyrum tataricum among and within the population was performed with simple sequence repeat (SSR) markers. The 15 SSR primer pairs were used for 75 individuals. Out of 15 primer pairs 7 primer pairs revealed 88.08%polymorphism among the population. The average gene diversity (h) among the populations was estimated to be 0.2098. Analysis of Molecular variance showed that the genetic variation (83.49%) was found mainly among the population. The coefficient of population differentiation Gst (0.8894) indicated that the variation existed among population, followed by the limited gene flow (N_m) 0.062. In the study, SSR markers showed high average polymorphic information content (0.93), marker ratio (0.36) and marker index (7.38). The genetic diversity, Shannon Index and percentage polymorphic band of F. tataricum significantly correlated with altitude. The genetic diversity among population of F. tataricum showed broad genetic base and provided the developmental and conservational strategies for crop improvement.