A cysteine-appended deoxyuridine for the postsynthetic DNA modification using native chemical ligation

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• 作者：Takeda ; Shuji ; Tsukiji ; Shinya ; Nagamune ; Teruyuki
• 关键词：Native chemical ligation ; Intein ; Deoxyuridine ; DNA
• 刊名：Tetrahedron Letters
• 出版年：2005
• 期刊代码：109_00404039
• 类别：ch
• 出版时间：March 28, 2005
• 卷：46
• 期：13
• 页码：2235-2238
• 文件大小：127 K

摘要

A new deoxyuridine derivative 6 bearing a cysteine group at the C5 position was synthesized and incorporated into oligodeoxynucleotide (ODN) by phosphoramidite chemistry. The postsynthetic DNA modification of the cysteine-containing ODN using native chemical ligation with thioesters of biotin and green fluorescent protein variant was successfully demonstrated.