Morphine Treatment Affects the Regulation of High Mobility Group I-type Chromosomal Phosphoproteins in C6 Glioma Cells

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• 作者：Chakarov ; Stojan ; Chakalova ; Lubomira ; Tencheva ; Zvetana ; Ganev ; Varban ; Angelova ; Anelia
• 关键词：high mobility group I-type proteins ; morphine ; C6 glioma cells
• 刊名：Life Sciences
• 出版年：2000
• 期刊代码：62_00243205
• 类别：imm
• 出版时间：2000
• 卷：66
• 期：18
• 页码：1725-1731
• 文件大小：328 K

摘要

High mobility group (HMG) I-type chromosomal phosphoproteins HMG I/Y and HMG I-C were investigated following morphine treatment of C6BU-1 glioma. Cells were labelled with [³²P]-orthophosphoric acid. Electrophoretic profiles and autoradiograms of the control cells revealed the presence of HMG I and HMG I-C proteins. HMG Y was not detected. Northern blot analysis showed a single HMG I/Y transcript. Treatment with morphine lowered the [³²P]-incorporation in HMG I and HMGI-C proteins and the level of the HMG I/Y transcript. However, it did not change the protein ratios on the Coomassie stained gels. These results suggest that morphine may trigger independent reaction pathways affecting either transcription regulation and/or postsynthetic phosphorylation of the preexisting HMG I-type proteins. In addition, opposing changes in the postsynthetic phosphorylation of HMG 14 and histones HI AB were also noticed.