The expression patterns of
PPARx3b2;/δ have been described, but the majority of these data are based on mRNA data. To date, there are no reports that have quantitatively examined the expression of PPAR
x3b2;/δ protein in mouse tissues. In the present study, a highly specific PPAR
x3b2;/δ antibody was developed, characterized, and used to examine tissue expression patterns of PPAR
x3b2;/δ. As compared to commercially available anti-PPAR
x3b2;/δ antibodies, one of six polyclonal anti-PPAR
x3b2;/δ antibodies developed was significantly more effective for immunoprecipitation of in vitro-translated PPAR
x3b2;/δ. This antibody was used for quantitative Western blot analysis using radioactive detection methods. Expression of PPAR
x3b2;/δ was highest in colon, small intestine, liver, and keratinocytes as compared to other tissues including heart, spleen, skeletal muscle, lung, brain, and thymus. Interestingly, PPAR
x3b2;/δ expression was localized in the nucleus and RXR
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can be co-immunoprecipitated with nuclear PPAR
x3b2;/δ. Results from these studies demonstrate that PPAR
x3b2;/δ expression is highest in intestinal epithelium, liver, and keratinocytes, consistent with significant biological roles in these tissues.