• 作者：Julio Torrado^a ; Marí ; a Blanca Piazuelo^b ; Irune Ruiz^c ; Marí ; a Isabel Izarzugaza^d ; Marí ; a Constanza Camargo^b ; Alberto Delgado^b ; Afshin Abdirad^e ; Pelayo Correa^b ; ^{pelayo.correa@vanderbilt.edu}
• 关键词：Esó ; fago de Barrett ; Mucinas ; MUC2 ; MUC5AC ; MUC6
• 刊名：Revista Espa帽ola de Patolog铆a
• 出版年：2010
• 期刊代码：pca318_16998855
• 类别：med
• 出版时间：January-March 2010
• 卷：43
• 期：1
• 页码：8-15
• 文件大小：895 K

Background

Barrett's esophagus is a known precursor lesion of esophageal adenocarcinoma. Although it is generally associated with gastroesophageal reflux, the pathogenic mechanisms of the disease are not well understood. The aim of this study was to explore the natural history and to identify markers of progression of the precancerous process.

Material and methods

Histological sections of 67 esophageal specimens were used in this study. They were obtained from 14 subjects with Barrett's esophagus who were followed from 1 to 9 years. The lesions were histologically classified as: Barrett's esophagus without dysplasia, indefinite for dysplasia, or dysplasia. Expression of various mucins in goblet and columnar cells was assessed by histochemistry and immunohistochemistry.

Results

Incomplete intestinal metaplasia was observed in all the specimens. Columnar cells within the metaplastic epithelium expressed neutral mucins. Sialomucins were significantly less expressed in columnar cells as the lesions increased in severity (p trend=0.03). Subjects with indefinite dysplasia lesions had significantly higher expression of sulphomucins in goblet cells (p=0.034) and of MUC2 in columnar cells (p=0.029) than subjects with Barrett's esophagus without dysplasia. Expression of the intestinal mucin MUC2 and gastric mucin MUC5AC was observed in all specimens. MUC6, a mucin of the deep gastric glands, was occasionally expressed.

Conclusion

The evaluation of the mucin profiles in Barrett's esophagus suggests a gradual transition of the metaplastic epithelium phenotype as the lesion advances in time.