Economical production of poly(纬-glutamic acid) using untreated cane molasses and monosodium glutamate waste liquor by Bacillus subtilis NX-2

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• 作者：Dan Zhang ; Xiaohai Feng ; Zhe Zhou ; Yang Zhang ; Hong Xu ; ^{xuh@njut.edu.cn}
• 关键词：Poly(纬-glutamic acid) ; Cane molasses ; Monosodium glutamate waste liquor ; Bacillus subtilis NX-2
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：June, 2012
• 卷：114
• 期：Complete
• 页码：583-588
• 文件大小：513 K

摘要

The production of poly(纬-glutamic acid) by Bacillus subtilis NX-2 from cane molasses and monosodium glutamate waste liquor (MGWL) was studied for the first time in this work. When batch fermentation was carried out with untreated molasses, 33.6 卤 0.37 g L^鈭? PGA was obtained with a productivity of 0.46 卤 0.006 g L^鈭? h^鈭?. In order to minimize the substrate inhibition, fed-batch fermentation was performed with untreated or hydrolyzed molasses in 7.5 L bioreactor, giving 50.2 卤 0.53 and 51.1 卤 0.51 g L^鈭? of PGA at 96 h, respectively. Further studies were carried out by using MGWL as another carbon source, resulting in a PGA concentration of 52.1 卤 0.52 g L^鈭? with a productivity of 0.54 卤 0.003 g L^鈭? h^鈭?. These results suggest that the low-cost cane molasses and MGWL can be used for the environmental-friendly and economical production of PGA by B. subtilis NX-2.