Proteomic analysis of early seed development in Pinus massoniana L

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• 作者：Yan Zhen^a ; Zhen-Zhou Zhao^a ; Ren-Hua Zheng^b ; Jisen Shi^a ; ^{jshi@njfu.edu.cn}
• 关键词：Embryogenesis ; Difference in-gel electrophoresis ; Masson pine ; Proteomics ; Seed development
• 刊名：Plant Physiology and Biochemistry
• 出版年：2012
• 期刊代码：87_09819428
• 类别：abs
• 出版时间：May, 2012
• 卷：54
• 期：Complete
• 页码：97-104
• 文件大小：691 K

摘要

Understanding seed development is important for large-scale propagation and germplasm conservation for the Masson pine. We undertook a proteomic analysis of Masson pine seeds during the early stages of embryogenesis. Two-dimensional difference gel electrophoresis (2D DIGE) was used to quantify the differences in protein expression during early seed development. Using electrospray ionization mass spectrometry/mass spectrometry, we identified proteins from 43 gel spots that had been excised from preparative 鈥減ick鈥?gels. Proteins involved in carbon metabolism were identified and were predominantly expressed at higher levels during the cleavage polyembryony and columnar embryo stages. Functional annotation of one seed protein revealed it involvement in programmed cell death and translation of selective mRNAs, which may play an important role in subordinate embryo elimination and suspensor degeneration in polyembryonic seed gymnosperms. Other identified proteins were associated with protein folding, nitrogen metabolism, disease/defense response, and protein storage, synthesis and stabilization. The comprehensive protein expression profiles generated by this study will provide new insights into the complex developmental process of seed development in Masson pine.