Proteomics-based identification of 伪-enolase as a potential prognostic marker in cholangiocarcinoma

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• 作者：Ponlapat Yonglitthipagon^a ; ^d ; Chawalit Pairojkul^a ; ^c ; Vajarabhongsa Bhudhisawasdi^b ; ^c ; Jason Mulvenna^d ; Alex Loukas^d ; Banchob Sripa^a ; ^c ; ^{ponlagon@gmail.com} ; ^{banchob@kku.ac.th}
• 关键词：Cholangiocarcinoma ; Proteomics ; Prognostic marker ; 伪-Enolase ; ENO1
• 刊名：Clinical Biochemistry
• 出版年：2012
• 期刊代码：55_00099120
• 类别：med
• 出版时间：July, 2012
• 卷：45
• 期：10-11
• 页码：827-834
• 文件大小：1309 K

摘要

Objectives

To investigate the association of expression status of 伪-enolase (ENO1) and clinicopathological outcomes of CCA patients.

Design and methods

Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to compare differential expressed protein profiles of four human CCA cell lines and H69, a non-malignant biliary cell line, as a control. Immunohistochemical analysis was carried out in tissue-microarray of human CCA tissues (n = 301).

Results

We identified ENO1 in all CCA cell lines but not H69 by proteomics based. About 75%of patients with CCA showed over-expression of ENO1 in hyperplastic bile duct and the tumors compared with that in tumor-adjacent normal tissue counterparts. Moreover, over-expression of ENO1 is significantly associated with poor prognosis and tumor invasion of CCA patients.

Conclusions

ENO1 may serve as a prognostic marker to monitor the disease progression of these patients.