In vivo tumor imaging using a novel RNAi-based detection mechanism

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• 作者：Kayiu Wan ; PhD^a ; Bernd Ebert ; PhD^b ; Jan Voigt ; PhD^b ; Qing Wang ; PhD^a ; Yiyang Dai ; MD^a ; Rainer Haag ; PhD^c ; Wolfgang Kemmner ; PhD^a ; ^{wkemmner@mdc-berlin.de}
• 关键词：Protoporphyrin-IX ; Silencing of ferrochelatase ; Molecular imaging
• 刊名：Nanomedicine ; Nanotechnology ; Biology and Medicine
• 出版年：2012
• 期刊代码：11_15499634
• 类别：et
• 出版时间：May, 2012
• 卷：8
• 期：4
• 页码：393-398
• 文件大小：1101 K

摘要

A new concept of tumor imaging is introduced using a siRNA-based probe that is capable of amplifying a specific endogenous fluorescence emission in cancerous tissue. In previous studies, we demonstrated a significant downregulation of Ferrochelatase (FECH) mRNA-expression in colorectal carcinomas leading to the accumulation of protoporphyrin IX (PpIX), a fluorescent metabolite of the heme synthesis. In this article, we report on first in vivo experiments in xenografted nude mice using folate-coupled liposomes or dendritic polyglycerolamine nanoparticles carrying ferrochelatase-siRNA to enhance PpIX-derived fluorescence in the tumor tissue. Tiny tumor foci could be monitored by the emission of PpIX fluorescence in vivo. Due to the omnipresence of the heme synthesis pathway, targeted application of ferrochelatase-siRNA may provide a general means for molecular imaging.

From the Clinical Editor

A new concept of tumor imaging is presented in this paper using a siRNA-based probe detecting protoporphyrin IX (PpIX), a fluorescent metabolite of the heme synthesis previously demonstrated to accumulate in cancer tissue.