Screening serum biomarker of knee osteoarthritis using a phage display technique

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• 作者：Xiaojun Weng ; Qiande Liao ; ^{sunshinexxus@yahoo.com} ; Kanghua Li ; Yong Li ; Meng Mi ; Da Zhong
• 关键词：COMP ; serum cartilage oligomeric matrix protein ; IgG ; Immunoglobulin G ; NPV ; negative predictive value ; PEG ; polyethanol glycol ; PPV ; positive predictive value ; OA ; osteoarthritis ; RA ; rheumatoid arthritis
• 刊名：Clinical Biochemistry
• 出版年：2012
• 期刊代码：55_00099120
• 类别：med
• 出版时间：March, 2012
• 卷：45
• 期：4-5
• 页码：303-308
• 文件大小：315 K

摘要

Objectives

To screen serum biomarker of knee osteoarthritis (OA) using a phage random peptide library.

Design and methods

A phage random peptide library of random peptide 12-mers was screened with purified immunoglobulin G (IgG) from sera of knee OA patients. Patients with knee rheumatoid arthritis (RA), hip OA, non-erosive hand OA or erosive hand OA, and healthy volunteers were used as controls.

Results

A phage clone with inserted peptide TGLESGHGPGDS (named KOA1) showed 90%positive reaction rate with the knee OA patients, significantly higher than that with the knee RA patients (27.8%), the non-erosive hand OA patients (34.3%), the erosive hand OA patients (31.3%) and the healthy controls (12.0%), but not the hip OA patients (82.5%).

Conclusions

The novel knee OA mimic peptide KOA1 identified with a random phage display peptide library and sera from knee OA patients could be a potential serum biomarker for knee OA.