Metabolic acidosis stimulates intestinal Glutamine absorption

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• 作者：Epler ; Mark J. ; Souba ; Wiley W. ; Meng ; QingHe ; Lin ; ChengMao ; Karinch ; Anne M. ; Vary ; Thomas C. ; et. al.
• 关键词：Glutamine ; intestine ; epidermal growth factor ; mitogen-activated protein kinase
• 刊名：Journal of Gastrointestinal Surgery
• 出版年：2003
• 期刊代码：159_1091255x
• 类别：med
• 出版时间：December 1, 2003
• 卷：7
• 期：8
• 页码：1045-1052
• 文件大小：254 K

摘要

Glutamine is an essential nutrient for cell integrity during acidotic states such as shock, but the effect of extracellular pH on intestinal mucosal cell glutamine uptake is poorly understood. The purpose of this in vitro study was to investigate the intracellular signaling pathways involved in controlling intestinal glutamine transport during acidosis. Lowering the pH in the cell culture medium resulted in an increase in glutamine transport activity in a time- and pH-dependent fashion. Chronic acidosis (pH 6.6 for 48 hours) resulted in a twofold increase in glutamine transport activity (1.63±0.25 nmole/mg protein/minute in acidosis vs. 0.78±0.11 nmole/mg protein/minute in control) and a threefold increase in glutamine transport gene ATB⁰ messenger RNA levels. This acidosis-induced increase in glutamine transport activity was due to a stimulation of transporter maximal transport capacity (V_max 13.6±0.73 nmole/mg protein/minute in acidosis vs. 6.3±0.46 nmole/mg protein/minute in control) rather than a change in transporter affinity (K_m