Colorimetric detection of cholesterol with G-quadruplex-based DNAzymes and ABTS^2鈭?/sup>

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• 作者：Ruimin Li ; Cen Xiong ; Zhiyou Xiao ; Liansheng Ling ; ^{cesllsh@mail.sysu.edu.cn}
• 关键词：Cholesterol ; Colorimetric detection ; DNAzyme ; ABTS2&minus
• 刊名：Analytica Chimica Acta
• 出版年：2012
• 期刊代码：2_00032670
• 类别：ch
• 出版时间：29 April, 2012
• 卷：724
• 期：Complete
• 页码：80-85
• 文件大小：600 K

摘要

A novel colorimetric method for detection of cholesterol was developed with hemin-G-quadruplex DNAzyme by transducing oxidation of cholesterol into the color change of 2,2鈥?azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS^{2鈭?/sup>). Oligonucleotide 5鈥?GTGGGTAGGGCGGGTTGG-3鈥?(Oligo-1) formed G-quadruplex structure in the presence of K+, it acted as a horseradish peroxidase (HRP) mimicking DNAzyme when binding hemin and catalyzed the oxidation of colorless ABTS2鈭?/sup> to green ABTS鈭?/sup> by H₂O₂, which was produced by the reaction of cholesterol and oxygen that catalyzed by cholesterol oxidase. Therefore, the oxidation of cholesterol could be transduced into the color change of ABTS2鈭?/sup> by combining these two reactions. Under the optimum conditions, the absorbance was proportional to the concentration of cholesterol over the range of 1.0-30 渭M, with a linear regression equation of A = 0.362 + 0.0256C (C: 渭M, R = 0.998) and a detection limit of 0.10 渭M (3蟽/slope). Moreover, the practicability of the assay in the detection of cholesterol in human serum was studied as well.}