Implication of hypR in the virulence and oxidative stress response of Enterococcus faecalis

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• 作者：Nicolas Verneuil ; Alain Rincé ; Maurizio Sanguinetti ; Yanick Auffray ; Axel Hartke and Jean-Christophe Giard
• 刊名：FEMS Microbiology Letters
• 出版年：2005
• 期刊代码：34_03781097
• 类别：imm
• 出版时间：2005
• 卷：252
• 期：1
• 页码：137-141
• 文件大小：212 K

摘要

HypR has recently been described as the first transcriptional regulator involved in the oxidative stress response and in the intracellular survival of Enterococcus faecalis within macrophages. In order to characterize the HypR regulon, real-time quantitative RT-PCR experiments were performed. The expression of four genes involved in the oxidative stress response encoding catalase, glutathione reductase, and the two subunits of alkyl hydroperoxide reductase were down regulated in the hypR background under H₂O₂ condition. These findings show that HypR acts as a transcriptional activator, especially during oxidative stress. In addition, DNAse I footprinting assays allowed us to identify the HypR-protected DNA regions corresponding to the “HypR box” in the hypR promoter. Moreover, the effect of the hypR mutation on the virulence of E. faecalis was evaluated in comparison with the wild-type JH2-2 strain using a mouse peritonitis model. Our results revealed that HypR appears to be an important virulence factor in E. faecalis.