Polyamines induce aggregation of LHC II and quenching of fluorescence in vitro

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• 作者：Theodoros Tsiavos ; Nikolaos E. Ioannidis¹ ; Kiriakos Kotzabasis ; ¹ ; ^{kotzab@biology.uoc.gr}
• 关键词：LHC II ; light-harvesting complex II ; Chl ; chlorophyll ; Neo ; neoxanthin ; Vio ; violaxanthin ; PAs ; polyamines ; Spm ; spermine ; Spd ; spermidine ; Put ; putrescine ; 螖pH ; thylakoid proton gradient ; qE ; 螖pH-dependent quenching of chlorophyll fluorescence ; DM ; dod
• 刊名：Biochimica et Biophysica Acta (BBA)/Bioenergetics
• 出版年：2012
• 期刊代码：13_00052728
• 类别：bio
• 出版时间：May, 2012
• 卷：1817
• 期：5
• 页码：735-743
• 文件大小：881 K

摘要

Dissipation of excess excitation energy within the light-harvesting complex of Photosystem II (LHC II) is a main process in plants, which is measured as the non-photochemical quenching of chlorophyll fluorescence or qE. We showed in previous works that polyamines stimulate qE in higher plants in vivo and in eukaryotic algae in vitro. In the present contribution we have tested whether polyamines can stimulate quenching in trimeric LHC II and monomeric light-harvesting complex b proteins from higher plants. The tetramine spermine was the most potent quencher and induced aggregation of LHC II trimers, due to its highly cationic character. Two transients are evident at 100 渭M and 350 渭M for the fluorescence and absorbance signals of LHC II respectively. On the basis of observations within this work, some links between polyamines and the activation of qE in vivo is discussed.