Intracellular interactions between protein 4.1 and glycophorin C on transport vesicles, as determined by fluorescence correlation spectroscopy

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• 作者：Shotaro Tanaka ; Yuichi Takakuwa ; ^{takakuwa@research.twmu.ac.jp}
• 关键词：FCS ; fluorescence correlation spectroscopy ; FCCS ; fluorescence cross-correlation spectroscopy ; LSM ; laser scanning microscopy ; D ; diffusion coefficient ; 4.1R ; protein 4.1R ; 4.1R30 ; protein 4.1R 30  ; kDa ; GPC ; glycophorin C ; TMD ; transmembrane domain ; T
• 刊名：FEBS Letters
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：23 March, 2012
• 卷：586
• 期：6
• 页码：668-674
• 文件大小：1068 K

摘要

Interaction of protein 4.1 (4.1R) with the transmembrane protein glycophorin C (GPC) regulates the functions of erythrocyte membrane. Fluorescence correlation spectroscopy (FCS) was used to define the interaction of EGFP-4.1R with DsRed-GPC on transport vesicles (TVs) by measuring their fluctuation in living cells. DsRed-GPC expressed in HeLa cells was delivered to the plasma membrane through slow vesicle transport. EGFP-4.1R, which freely diffused in the cytosol when expressed alone, diffused slowly when co-expressed with DsRed-GPC, indicating association of EGFP-4.1R with TVs. Fluorescence cross-correlation spectroscopy (FCCS) showed direct interaction of EGFP-4.1R with DsRed-GPC on TVs. The present study demonstrates that 4.1R binds to GPC on TVs in living cells.4 class="h4">Structured summary of protein interactions4>

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