Post-transcriptional modulation of C/EBP伪 prompts monocytic differentiation and apoptosis in acute myelomonocytic leukaemia cells

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• 作者：Hideki Yoshida^a ; Toshihiko Imamura^a ; ^{imamura@koto.kpu-m.ac.jp} ; Atsushi Fujiki^a ; Yoshifumi Hirashima^a ; Mitsuru Miyachi^a ; Takeshi Inukai^b ; Hajime Hosoi^a
• 关键词：Acute myeloid leukaemia ; C/EBP伪 ; RAD001 ; p42/p30 ratio ; Dephosphorylation ; mTOR ; Apoptosis
• 刊名：Leukemia Research
• 出版年：2012
• 期刊代码：186_01452126
• 类别：med
• 出版时间：June, 2012
• 卷：36
• 期：6
• 页码：735-741
• 文件大小：1087 K

摘要

CCAAT/enhancer binding protein alpha (C/EBP伪) induction induces monocytic differentiation even in acute myeloid leukaemia (AML). In this study, the induction/activation of C/EBP伪 in myelomonocytic AML was investigated using a combination of all-trans retinoic acid (ATRA) and RAD001 (Everolimus), a mammalian target of rapamycin complex 1 (mTORC1) inhibitor. Combining these agents increased PU.1, C/EBP蓻 and C/EBP伪 expression, increased the p42/p30 C/EBP伪 ratio, and decreased C/EBP伪 phosphorylation at serine 21, and was accompanied by growth inhibition, induction of CD11b expression and apoptosis in AML cell lines. Thus, agents that induce sufficient levels of C/EBP伪 expression might be useful in treating AML.