Sensitive detection of carcinoembryonic antigen with magnetic nano-bead and upconversion nanoparticles-based immunoassay

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• 作者：Zhaohui Huang^a ; ^{hzhwxsy@126.com} ; Shijia Wu^b ; Nuo Duan^b ; Dong Hua^a ; Yu Hu^a ; Zhouping Wang^b ; ^{wangzp@jiangnan.edu.cn}
• 关键词：CEA ; carcinoembryonic antigen ; MNP ; magnetic nanoparticle ; UCNPs ; upconversion nanoparticles ; ELISA ; enzyme-linked immunosorbent assays ; RIA ; radioimmunoassays ; PBS ; phosphate-buffered saline ; XRD ; X-ray diffraction ; TEM ; transmission electron microscope
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2012
• 期刊代码：65_07317085
• 类别：ch
• 出版时间：July, 2012
• 卷：66
• 期：Complete
• 页码：225-231
• 文件大小：768 K

摘要

A novel magnetic nanobead-based immunoassay was developed for the quantification of carcinoembryonic antigen (CEA) in human serum in this study. Amine-functionalized Fe₃O₄ magnetic nanoparticles (MNPs) were conjugated with capture anti-CEA antibodies and amine-functionalized NaY_0.78F₄:Yb_0.20, Ho_0.02 upconversion nanoparticles (UCNPs) were synthesized and conjugated with detection anti-CEA antibodies, respectively. Based on a sandwich-type immunoassay format, the detection limit for CEA under optimal conditions was as low as 2.5 pg/mL, and the linear range of CEA detection was from 2.5 脳 10^鈭?2 to 1 脳 10^鈭? g/mL (I = 406.19 log X + 428.81) with a correlation coefficient equal to 0.9987. The established method was successfully applied to measure CEA in human serum samples and more sensitive than a commercially available chemiluminescence method. The precision expressed as the relative standard deviation of CEA detection was equal to 5.19%(1 ng/mL) or 4.36%(5 pg/mL), indicating that the developed method exhibited good reproducibility. The results demonstrate that the method offers potential advantages of sensitivity and good reproducibility for the determination of CEA, and is applicable to the determination of CEA in serum samples.