HMGB-1 induces cell motility and 伪5尾1 integrin expression in human chondrosarcoma cells

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• 作者：Chih-Hsin Tang^a ; ^b ; ^{chtang@mail.cmu.edu.tw} ; Yun-Ting Keng^c ; Ju-Fang Liu^d
• 关键词：HMGB-1 ; high mobility group box chromosomal protein 1 ; RAGE ; receptor for advanced glycation end products ; PI3K ; phosphatidylinositol 3-kinase ; ECM ; extracellular matrix ; siRNA ; small interfering RNA ; qPCR ; quantitative real time PCR
• 出版年：2012
• 期刊代码：44_03043835
• 类别：med
• 出版时间：1 September, 2012
• 卷：322
• 期：1
• 页码：98-106
• 文件大小：1010 K

摘要

Chondrosarcoma is a type of highly malignant tumor with a potent capacity to invade locally and cause distant metastasis. High mobility group box chromosomal protein 1 (HMGB)-1 is a widely studied, ubiquitous nuclear protein that is present in eukaryotic cells, and plays a crucial role in inflammatory response. However, the effects of HMGB-1 on human chondrosarcoma cells are largely unknown. In this study, we found that HMGB-1 increased the migration and the expression of 伪5尾1 integrin in human chondrosarcoma cells. Transfection of cells with receptor for advanced glycation end products (RAGE) receptor siRNA reduced HMGB-1-induced cell migration and integrin expression. Activations of phosphatidylinositol 3-kinase (PI3K), Akt, and AP-1 pathways after HMGB-1 treatment were demonstrated, and HMGB-1-induced expression of integrin and migration activity was inhibited by the specific inhibitor and mutant of PI3K, Akt, and AP-1 cascades. Taken together, our results indicated that HMGB-1 enhances the migration of chondrosarcoma cells by increasing 伪5尾1 integrin expression through the RAGE receptor/PI3K/Akt/c-Jun/AP-1 signal transduction pathway.