l-2-oxothiazolidine-4-carboxylate influence on age- and heat exposure-dependent peroxidation in rat's liver and kidney

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• 作者：Nikola Hadzi-Petrushev^a ; Nikola Jankulovski^b ; Mishko Milev^a ; Pavlina Filipovska^a ; Hristo Gagov^c ; Elizabeta Gjorgievska^d ; Dine Mitrov^e ; Ramadan Sopi^a ; Kiril Hristov^c ; Mitko Mladenov^a ; ^c ; ^{mitkom@pmf.ukim.mk}
• 关键词：ROS ; reactive oxygen species ; OTC ; l-2-oxothiazolidine-4-carboxylate ; GSH ; glutathione ; GPx ; glutathione peroxidase ; GR ; glutathione reductase ; CAT ; catalase ; H2O2 ; hydrogen peroxide ; Tco ; colorectal temperature ; GSSG ; glutathione disulfide ; HO ; hydroxyl
• 刊名：Journal of Thermal Biology
• 出版年：2012
• 期刊代码：100_03064565
• 类别：bio
• 出版时间：August, 2012
• 卷：37
• 期：5
• 页码：361-365
• 文件大小：444 K

摘要

To investigate the impact of acute heat exposure on maintenance of redox homeostasis and antioxidant balance related to aging, we have determined the GSH levels in the liver and kidney, and the activity of antioxidant enzymes in the same organs from Wistar rats at two different ages, 35 days and 18 months. The animals were housed individually in a special heated chamber maintaining a constant temperature of 40卤0.5 掳C. The results showed that the level of endogenous GSH was signi铿乧antly lower in aged than in young animals. In general, the activity of antioxidant enzymes in investigated tissues displayed an age-dependent decline. Indeed, we found unchanged CAT activity and decreased GPx activity with age. On the other hand acute heat exposure led to disproportion between peroxide metabolizing enzymes (CAT, GPx) and GR, thus promoting H₂O₂ accumulation and prooxidative state in the liver of young animals. The results for the impact of l-2-oxothiazolidine-4-carboxylate in combined stress model suggested that in spite of restore levels of GSH, the restoration of oxido-reductive balance might have only been partial due to irreversible alterations in antioxidant enzymes set by acute heat exposure and aging. Interestingly, young animals appeared to be more sensitive to the supplementation of the l-2-oxothiazolidine-4-carboxylate, likely because of the more extensive increase of GSH observed in young l-2-oxothiazolidine-4-carboxylate treated animals.