• 作者：M. Ló ; pez-Laguna^a ; ^{martalopez@bantecyl.es} ; A. Saldañ ; a-Dí ; az^b ; L. Rafael Ramos-Pascua^b ; J.A. Rodrí ; guez-Altó ; naga-Martí ; nez^c ; J. Ramos-Carro^a ; P. de la Puente Garcí ; a^a ; J.J. Vá ; zquez-Reyero^a ; J.Á ; . Santos-Sá ; nchez^d ; F.J. Iglesias-Muñ ; oz^a
• 关键词：Cé ; lulas madre ; Ingenierí ; a tisular ; Traumatologí ; a ; Resonancia magné ; tica
• 刊名：Revista Espa帽ola de Cirug铆a Ortop茅dica y Traumatolog铆a
• 出版年：2011
• 期刊代码：pca311_18884415
• 类别：med
• 出版时间：September-October 2011
• 卷：55
• 期：5
• 页码：369-377
• 文件大小：459 K

lass="h4">Objective

The aim of this study was to study the viability and multipotential capacity of adipose derived mesenchymal stem cells, after being labelled with superparamagnetic iron oxide particles. The final aim is to monitor stem cells after being implanted by cellular therapy and tissue engineering techniques in the field of Orthopaedics and Traumatology.

lass="h4">Method

Ten biopsies of adipose tissue were processed in order to isolate adipose derived mesenchymal stem cells, which were cultured under standard in vitro culture conditions until pre-confluent state. Cells in 1P and 2P were labelled with superparamagnetic iron oxide particles (SPIO), using protamine sulphate as a transfection agent. After confirming labelling efficiency with Prussian blue staining, we studied the labelling effect on the growth and multipotency of stem cells by analysing their in vitro growth curves and their capacity to differentiate into adipogenic, osteogenic and chondrogenic cell lines. We also tried to view the labelled stem cells using 3 Tesla magnetic resonance imaging.

lass="h4">Results and conclusion

Adipose derived stem cells labelled with SPIO showed a proliferation and differentiation capacity similar to control stem cells. These cells were visible on MR images after been implanted in a bone piece. Therefore, in vitro cell labelling of adipose derived mesenchymal stem cells with SPIO could be useful for in vivo cell tracking by MRI in bone tissue engineering.