GABA (A) receptor subunits RNA expression in mice peritoneal macrophages modulate their IL-6/IL-12 production
- 作者：Maria Guadalupe Reyes-Garcí ; a ; Francisca Herná ; ndez-Herná ; ndez ; Beatriz Herná ; ndez-Té ; llez ; Fern ; o Garcí ; a-Tamayo
- 关键词：Macrophages ; GABA ; IL-6 ; IL-12 ; GABA A receptor ; RT-PCR
- 刊名：Journal of Neuroimmunology
- 出版年：2007
- 期刊代码：32_01655728
- 类别：neu
- 出版时间：August 2007
- 卷：188
- 期：1-2
- 页码：64-68
- 文件大小：620 K
摘要
The expression and functionality of the gamma aminobutyric acid A receptor (GABAAR) in mice macrophages was explored. Reverse Transcriptase-Polymerase Chain Reaction showed that macrophages express the GABAAR RNA for the α1, α2, β3 and δ subunits, but not for the α5, β1, β2 and γ3 subunits. The expression of α1 subunit was also revealed by confocal microscopy. LPS-stimulated macrophages significantly reduced their in vitro production of IL-6 and IL-12 after GABA adding to the culture medium. These results showed that BALB/c mice peritoneal macrophages express a functional subset of GABAAR subunits.