Administration of recombinant Reishi immunomodulatory protein (rLZ-8) diet enhances innate immune responses and elicits protection against nervous necrosis virus in grouper Epinephelus coioides

详细信息	查看全文 | 推荐本文 |

• 作者：Yen-Chou Kuan^a ; ¹ ; Fuu Sheu^a ; ^b ; ¹ ; Guo-Chi Lee^c ; Ming-Wei Tsai^d ; Chih-Liang Hung^a ; Fan-Hua Nan^d ; ^{fhnan@mail.ntou.edu.tw}
• 关键词：Dietary administration ; Reishi immunomodulatory protein LZ-8 ; Epinephelus coioides ; Innate immunity ; Nervous necrosis virus
• 刊名：Fish & Shellfish Immunology
• 出版年：2012
• 期刊代码：115_10504648
• 类别：abs
• 出版时间：June, 2012
• 卷：32
• 期：6
• 页码：986-993
• 文件大小：689 K

摘要

Nervous necrosis virus (NNV) infection during larvae and juvenile stage in grouper (Epinephelus coioides) has caused severe economic losses in the aquaculture industry in Asia. The aims of this study were to evaluate the influence of recombinant Reishi protein, rLZ-8, on the innate immune responses and the viral resisting ability in fish. Groupers were fed with rLZ-8 supplemented diet (1.25-37.5聽mg _rLZ-8/kg _diet), and the cytokine gene expression, innate immune responses, and survival rate after NNV challenge were examined. The fish fed with rLZ-8 diet showed 6- to 11-fold upregulated TNF-伪 and IL-1尾 gene expression, along with significant increased respiratory burst and phagocytic activity. Moreover, feeding the fish with 37.5聽mg/kg rLZ-8 diet elicited significant improvement in post viral challenge survival rate (85.7%). These discoveries indicated that rLZ-8 could be utilized as an ant-pathogen immunostimulant, and provided a new candidate to fight against NNV infection in fish.