Preferential induction of Th17 cells in vitro and in vivo by Fucogalactan from Ganoderma lucidum (Reishi)

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• 作者：Hideyuki Yoshida^a ; ^b ; Mayu Suzuki^a ; ^b ; Ryota Sakaguchi^a ; ^b ; Ito Tani^a ; ^b ; Hitoshi Kotani^a ; ^b ; Norimasa Shudo^c ; Akihiko Yoshimura^a ; ^b ; ^{yoshimura@a6.keio.jp}
• 关键词：Interleukin ; ERK ; Dendritic cell ; 尾-Glucan ; Helper T cell
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：25 May, 2012
• 卷：422
• 期：1
• 页码：174-180
• 文件大小：864 K

摘要

The mushroom known as Reishi (Ganoderma lucidum) has been used as an herbal medicine for tumor treatment and immune system activation. Because its effects on the differentiation of effector T helper cells have not yet been fully understood, we investigated the effects of Reishi and those of its principal ingredient, 尾-glucan, on the activation of dendritic cells and the differentiation of Th17 cells. Reishi extracts as well as purified 尾-glucan (Curdran) activated DCs and caused them to produce large amounts of IL-23. 尾-glucan also enhanced and sustained the transcription of IL-23p19. The MEK-ERK signaling pathway positively regulates IL-23p19 transcription in 尾-glucan-stimulated DCs. In a mixed leukocyte reaction, Reishi-stimulated DCs preferentially induced Th17 cells. Furthermore, orally-administrated Reishi increased the percentages of Th17 cells and the transcription levels of antimicrobial peptides. Our results show that Reishi and 尾-glucan activate DCs to produce large amounts of IL-23, which induces Th17 differentiation both in vitro and in vivo.