Triphlorethol-A induces heme oxygenase-1 via activation of ERK and NF-E2 related factor 2 transcription factor
- 作者：Kyoung Ah Kang ; Kyoung Hwa Lee ; Jae Woo Park ; Nam Ho Lee ; Hye Kyung Na ; Young Joon Surh ; Ho Jin You ; Myung Hee Chung ; Jin Won Hyun
- 关键词：Triphlorethol-A ; Heme oxygenase-1 ; NF-E2 related factor 2 ; Antioxidant response element
- 刊名：FEBS Letters
- 出版年：2007
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：15 May 2007
- 卷：581
- 期：10
- 页码：2000-2008
- 文件大小：577 K
摘要
Triphlorethol-A, phlorotannin found in Ecklonia cava, induced heme oxygenase-1 (HO-1) expression at mRNA and protein levels, leading to increased HO-1 activity. Transcription factor NF-E2 related factor 2 (Nrf2) regulates antioxidant response element (ARE) of phase 2 detoxifying and antioxidant enzymes. Triphlorethol-A increased nuclear translocation, ARE binding, and transcriptional activity of Nrf2. Triphlorethol-A exhibited activation of ERK and U0126, inhibitor of ERK kinase, suppressed triphlorethol-A induced activation of Nrf2, finally decreased HO-1 protein level. Taken together, these data suggest that triphlorethol-A augments cellular antioxidant defense capacity through induction of HO-1 via ERK-Nrf2-ARE signaling pathway, thereby protecting cells from oxidative stress.