Genetic relatedness among Listeria monocytogenes isolated in foods and food production chain in southern Rio Grande do Sul, Brazil

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• 作者：Karla Sequeira Mendonç ; a^a ; ^{karlasmend@yahoo.com.br} ; Geovana Brenner Michael^b ; ¹ ; ^{geovana.michaelbrenner@fli.bund.de} ; Ana Eucares von Laer^a ; ² ; ^{ana.eucares@cafw.ufsm.br} ; Dulcinea Blum Menezes^c ; ^{dulcinea.blum@ufpel.edu.br} ; Marisa Ribeiro Itapema Cardoso^b ; ^{mcardoso@ufrgs.br} ; Wladimir Padilha da Silva^a ; ^{silvawp@ufpel.edu.br}
• 关键词：Listeria monocytogenes ; Food production chain ; Food ; Diversity ; Serotypes ; PFGE
• 刊名：Food Control
• 出版年：2012
• 期刊代码：49_09567135
• 类别：abs
• 出版时间：November, 2012
• 卷：28
• 期：1
• 页码：171-177
• 文件大小：372 K

摘要

Listeria monocytogenes is a foodborne bacterial pathogen of great concern to food industry. This is mainly due to its capacity to grow at low temperatures, its wide distribution in the environment and ability to adhere to various surfaces that come into contact with food. The aim of this study was to investigate the clonal relationship among L.聽monocytogenes isolates. Our purpose was to better understand the diversity of this pathogen in foods and food production chains in southern Rio Grande do Sul (Brazil). Forty four L.聽monocytogenes strains were characterized by serotyping and PFGE. Six different serotypes were found in the food and food environment (1/2a, 1/2b, 1/2c, 3a, 4b, 4e) and combination of macrorestriction patterns using AscI and ApaI, yielded 29 different pulsotypes. Strains with identical restriction patterns were isolated from foods of different sources and environments at different times. The presence of persistent strains of L.聽monocytogenes emphasizes the importance of cross-contamination in these food processing environments. It is likely that this occurs mainly due to ineffective cleaning and sanitization procedures, which allow for the survival and adaptation of these strains in the food processing environment, thereby causing persistence and contamination of final products.