Human gastric cells resistant to (−)-epigallocatechin gallate show cross-resistance to several environmental pollutants
- 作者：S.B. Horng ; H.H. Kuo ; M.Y. Lin ; W.W. Lin ; T.C. Wang
- 关键词：Resistance ; AGS cells ; EGCG ; Insecticide ; Oxidative stress
- 刊名：Food and Chemical Toxicology
- 出版年：2007
- 期刊代码：20_02786915
- 类别：pha
- 出版时间：November 2007
- 卷：45
- 期：11
- 页码：2171-2178
- 文件大小：386 K
摘要
After a long-term culture in (−)-epigallocatechin gallate (EGCG, 20 μM), a major constituent of green tea, human gastric AGS cells developed 2.2-fold resistance to EGCG. The resistant AGS (AGS-R) cells were cross-resistant to several N-methylcarbamate insecticides, which are among the major control agents for pest insects in Taiwan. The AGS-R cells also showed protective effects against both the cytotoxicity and DNA damage induced by one of the mutagenic derivatives of N-methylcarbamate insecticide, N-nitroso methomyl, which is known to target the mammalian gastric tract. Therefore, acquisition of resistance by AGS cells through chronic exposure to EGCG implies that the tea-drinking habit of the Taiwanese is probably beneficial for the health of the gastric tract. In addition, AGS-R cells were cross-resistant to sodium arsenite and hydrogen peroxide, indicating that tolerance to oxidative stress might play a role in the development of resistance described in this investigation.