• 作者：José ; M. Eiros^a ; ^{eiros@med.uva.es} ; Raú ; l Ortiz de Lejarazu^a ; Alberto Tenorio^a ; Inmaculada Casas^b ; Francisco Pozo^b ; Guillermo Ruiz^b ; Pilar Pé ; rez-Breñ ; a^b
• 关键词：Virus respiratorios ; Diagnó ; stico viroló ; gico ; Virus de la gripe ; Diagnó ; stico microbioló ; gico molecular
• 刊名：Enfermedades Infecciosas y Microbiolog铆a Cl铆nica
• 出版年：2009
• 期刊代码：pca153_0213005X
• 类别：med
• 出版时间：March 2009
• 卷：27
• 期：3
• 页码：168-177
• 文件大小：241 K

Acute respiratory infection is the most common disease occurring over a person's lifetime, with etiological variations determined mainly by age, environmental circumstances, the healthcare setting, and the underlying pathology. More than 200 different viruses distributed in six viral families have been implicated in the pathogenesis of respiratory tract infection. These facts are generating an increasing diagnostic demand that should be incorporated into the healthcare setting without delay. To meet this demand, the Spanish Society of Infectious Diseases and Clinical Microbiology has updated its Standard Procedure for the microbiological diagnosis of viral respiratory infection. This document contains an update primarily of infections caused by influenza viruses, and secondarily, infections due to other conventional and emerging respiratory viruses. In all cases, the methods for direct virological diagnosis (cell culture, and detection of antigens and nucleic acid) are reviewed, with special reference to techniques for molecular detection and genetic characterization.