Effect of a DNA vaccine harboring two copies of inhibin 伪 (1-32) fragments on immune response, hormone concentrations and reproductive performance in rats

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• 作者：Shui-Lian Wang^a ; ¹ ; ^{wangshuilian1234@yahoo.com.cn} ; Li Han^b ; S. Ahmad^b ; ^c ; Shao-Xian Cao^d ; Li-Qun Xue^a ; Zhao-Fang Xing^e ; Jian-Zhong Feng^f ; Ai-Xin Liang^b ; Li-Guo Yang^b ; ¹
• 关键词：Inhibin ; DNA vaccine ; Immune response ; Reproduction ; Hormone
• 刊名：Theriogenology
• 出版年：2012
• 期刊代码：98_0093691x
• 类别：abs
• 出版时间：15 July, 2012
• 卷：78
• 期：2
• 页码：393-401
• 文件大小：751 K

摘要

The objective was to investigate the effects of a novel DNA vaccine (pcISI) harboring two copies of inhibin 伪 (1-32) fragments on immune response, hormone concentrations and reproductive performance in rats. Female Wistar rats (n = 18 per group) were immunized (twice, 4 wk apart) with 10, 50, or 100 渭g (T₁, T₂ and T₃, respectively), of the pcISI plasmid. At 4 wk after the second immunization, plasma antibody titers were higher (P < 0.05) in T₃ than in either T₁ or T₂ (0.341 卤 0.123, 0.236 卤 0.068, and 0.251 卤 0.077, respectively, mean 卤 SD). Concurrrently, plasma concentrations of FSH and estradiol were highest (P < 0.05) in T₃, and were higher (P < 0.05) in T₁ and T₂ than in control groups. For antibody-positive rats, there was a correlation (P < 0.01) between antibody titer and FSH concentrations after two pcISI immunizations. The number of mature follicles in the T₃ group (46.00 卤 4.65) was higher (P < 0.05) than in two control groups (29.25 卤 3.72 and 27.92 卤 3.48), and also higher (P < 0.05) than in T₁ and T₂ (37.17 卤 4.99 and 38.75 卤 7.09). Antibody-positive rats had more mature ovarian follicles than negative rats (46.75 卤 4.23 vs. 35.60 卤 3.38, P < 0.05). Moreover, litter size and number of placentas were increased (P < 0.05) in the pcISI immunization groups, except for the T₁ group, compared to the control groups. In conclusion, the pcISI DNA vaccine successfully induced a humoral immune response, improved reproductive hormone concentrations, stimulated follicular development, and increased number of placentas and litter size. Furthermore, 100 渭g yielded the best immune response.