PKC未 and 蔚 regulate the morphological integrity of the ER-Golgi intermediate compartment (ERGIC) but not the anterograde and retrograde transports via the Golgi apparatus

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• 作者：Taichi Sugawara^a ; ¹ ; Daiki Nakatsu^a ; ¹ ; Hiroaki Kii^b ; Nobuhiko Maiya^b ; Atsuhiro Adachi^a ; Akitsugu Yamamoto^c ; Fumi Kano^a ; Masayuki Murata^a ; ^{mmurata@bio.c.u-tokyo.ac.jp}
• 关键词：ERGIC ; ER&ndash ; Golgi intermediate compartment ; ER ; endoplasmic reticulum ; VSVG ; vesicular stomatitis virus G-protein ; CtxB ; cholera toxin B subunit ; PMA ; phorbol 12-myristate 13-acetate ; FCCP ; carbonylcyanide-p-trifluoromethoxyphenylhydrazone ; TMRM ; tetr
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular Cell Research
• 出版年：2012
• 期刊代码：20_01674889
• 类别：bio
• 出版时间：April, 2012
• 卷：1823
• 期：4
• 页码：861-875
• 文件大小：3156 K

摘要

The ER-Golgi intermediate compartment (ERGIC) is an organelle through which cargo proteins pass and are being transferred by either anterograde or retrograde transport between the endoplasmic reticulum (ER) and the Golgi apparatus. We examined the effect of 80 different kinase inhibitors on ERGIC morphology and found that rottlerin, a PKC未 inhibitor, induced the dispersion of the perinuclear ERGIC into punctate structures. Rottlerin also delayed anterograde transport of vesicular stomatitis virus G protein (VSVG) from the ER to the Golgi and retrograde transport of cholera toxin from cell surface to the ER via the Golgi. RNA interference revealed that knockdown of PKC未 or 蔚 resulted in the dispersion of the ERGIC, but unexpectedly did not inhibit VSVG and cholera toxin transport. We also found that rottlerin depolarized the mitochondrial membrane potential, as does carbonylcyanide-p-trifluoromethoxyphenylhydrazone (FCCP), an uncoupler, and demonstrated that a decrease in the intracellular adenosine triphosphate (ATP) levels by rottlerin might underlie the block in transports. These results suggest that PKC未 and 蔚 specifically regulate the morphology of the ERGIC and that the maintenance of ERGIC structure is not necessarily required for anterograde and retrograde transports.