Characterisation and clonal dissemination of OXA-23-producing Acinetobacter baumannii in Tabriz, northwest Iran

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• 作者：Amir Peymani^a ; Paul G. Higgins^b ; ^{paul.higgins@uni-koeln.de} ; Mohammad-Reza Nahaei^c ; Safar Farajnia^d ; Harald Seifert^b
• 关键词：DiversiLab ; International clones ; Carbapenem resistance ; ISAba1
• 刊名：International Journal of Antimicrobial Agents
• 出版年：2012
• 期刊代码：126_09248579
• 类别：med
• 出版时间：June, 2012
• 卷：39
• 期：6
• 页码：526-528
• 文件大小：156 K

摘要

The characteristics and molecular epidemiology of carbapenemase genes amongst 68 imipenem-resistant Acinetobacter baumannii isolated from Imam Reza Hospital (Tabriz, Iran) during a 17-month period were studied. All 68 isolates were typed using sequence group-based multiplex polymerase chain reaction (PCR) to compare the clonal relationship of isolates with known international clonal lineages. Repetitive sequence-based PCR was further performed with representative isolates of each clone. PCR and sequencing were performed to detect OXA-type carbapenemases and class 1, 2 and 3 integron genes as well as to confirm the presence of insertion sequence ISAba1 upstream of bla_OXA-23 and bla_OXA-51-like genes. Sixty-four isolates (94%) belonged to international clone (IC) II, two isolates (3%) belonged to IC I and two isolates (3%) did not belong to known international clones. All isolates carried bla_OXA-51-like, bla_OXA-23 and class 1 integron genes. No other acquired bla_OXA genes or class 2 or 3 integron genes were detected. Sequence analysis confirmed the presence of bla_OXA-23 as well as the bla_OXA-51-like variants bla_OXA-66, bla_OXA-69 and bla_OXA-88. ISAba1 was present upstream of the bla_OXA-23 gene in all of the isolates. Clonal spread of OXA-23-producing A. baumannii emphasises the need for appropriate infection control measures to prevent further spread of these multidrug-resistant organisms.