Tissue engineering for full-thickness burns: A dermal substitute from bench to bedside
- 作者：Sabrina Kellouche ; Christophe Martin ; Gregory Korb ; Roger Rezzonico ; Delphine Bouard ; Marc Benbunan ; Louis Dubertret ; Christophe Soler ; Chantal Legr ; ; Christine Dosquet
- 关键词：Burns ; Dermal matrix ; Dermal substitute ; Foreskin fibroblasts ; Platelets ; VEGF
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：23 November 2007
- 卷：363
- 期：3
- 页码：472-478
- 文件大小：2920 K
摘要
Our aim was to obtain a viable and easily available dermal substitute (DS) for the definitive coverage of full-thickness burns. A DS composed of a collagen–glycosaminoglycan–chitosan dermal matrix (DM) colonized with foreskin fibroblasts (FF) is described. FF-colonized DS were compared to the DM seeded with adult dermal fibroblasts (DF). FF-colonized DS expressed more fibrillin and tropoelastin than that with DF. Reconstructed skin obtained with both FF- and DF-colonized DS similarly expressed laminin-5 and collagen VII at the dermal–epidermal junction. Both FF- and DF-colonized DS produced cutaneous wound healing mediators in a dose-dependent manner in the presence of platelet lysate. After freeze-thawing, the FF-colonized DS were recovered in culture and retained their ability to produce vascular endothelial growth factor. Grafting of DS into nude rats achieved a complete healing of a dermal–epidermal lesion with a good epidermalization.