Humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A

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• 作者：Ivo Alexandre Leme da Cunha^a ; Dauton Luiz Zulpo^a ; Alexey Leon Gomel Bogado^a ; Luiz Daniel de Barros^a ; Alessandra Taroda^a ; Michelle Igarashi^b ; Italmar Teodorico Navarro^a ; Joã ; o Luis Garcia^a ; ^{jlgarcia@uel.br}
• 关键词：Toxoplasma gondii ; Rhoptry ; Mucosal immunity ; Pigs
• 刊名：Veterinary Parasitology
• 出版年：2012
• 期刊代码：107_03044017
• 类别：abs
• 出版时间：25 May, 2012
• 卷：186
• 期：3-4
• 页码：216-221
• 文件大小：295 K

摘要

We evaluated the humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A. The experiment used 13 mixed-breed pigs divided into the following three groups: G1 (vaccinated-challenged, n = 6), which received the rhoptry vaccine (200 (g/dose); G2 (adjuvant-challenged, n = 4), which received PBS plus Quil-A; and G3 (unvaccinated-challenged, n = 3), which was the control group. The treatments were performed intranasally at days 0, 21, and 42. Three pigs from G1 produced IgG and IgM antibody levels above the cut-off in the ELISA on the challenge day. Partial protection was observed in G1 at the chronic phase of infection when compared with G3. The preventable fractions were 41.6%and 6.5%, in G1 and G2, respectively. The results of this study suggest that rhoptry proteins plus Quil-A stimulated humoral, local, and systemic immune responses, which were able to partially protect the brain from cyst formation.