C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses

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• 作者：Mohamad Alaa Terkawi^a ; Hend Alhasan^a ; Akio Ueno^a ; Jadsada Ratthanophart^a ; Yuzi Luo^a ; Shinuo Cao^a ; Ketsarin Kamyingkird^a ; Mahmoud Aboulaila^a ; ^b ; Goo Youn-Kyoung^a ; Yoshifumi Nishikawa^a ; Naoaki Yokoyama^a ; Xuenan Xuan^a ; Ikuo Igarashi^a ; ^{igarcpmi@obihiro.ac.jp}
• 关键词：Babesia caballi ; 48-kDa Rhoptry protein ; Diagnosis ; ELISA ; Immunochromatographic test
• 刊名：Parasitology International
• 出版年：2012
• 期刊代码：125_13835769
• 类别：bio
• 出版时间：September, 2012
• 卷：61
• 期：3
• 页码：493-496
• 文件大小：303 K

摘要

A recombinant C-terminal antigen derived from Babesia caballi 48-kDa rhoptry protein (rBc48/CT) was made for the development of a serologically diagnostic test. Antiserum raised against the rBc48/CT reacted specifically with the corresponding native protein by Western blotting and the indirect fluorescent antibody test (IFAT). Next, an indirect enzyme-linked immunosorbent assay (Bc48/CT-ELISA) and an immunochromatographic test based on the Bc48/CT (Bc48/CT-ICT) were constructed and employed for the detection of an antibody to B. caballi in a variety of equine sera. The results of Bc48/CT-ELISA and Bc48/CT-ICT were highly concordant with those of IFAT and ELISA, with full-length protein of Bc48 used as the reference tests. Our results demonstrate the success of Bc48/CT as antigen for the serological diagnosis of B. caballi infection in horses.