A dam mutant of Yersinia pestis is attenuated and induces protection against plague

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• 作者：Victoria L. Robinson ; Petra C.F. Oyston and Richard W. Titball
• 关键词：DNA adenine methylase ; Plague ; Vaccine
• 刊名：FEMS Microbiology Letters
• 出版年：2005
• 期刊代码：34_03781097
• 类别：imm
• 出版时间：2005
• 卷：252
• 期：2
• 页码：251-256
• 文件大小：151 K

摘要

We have constructed a dam mutant of Yersinia pestis GB. In BALB/c mice inoculated subcutaneously, the median lethal dose of the mutant was at least 2000-fold higher than the wild type. Mice inoculated with sub-lethal doses of the mutant were protected against a subsequent challenge with virulent Y. pestis. The effect of dam inactivation on gene expression was examined using a DNA microarray, which revealed increased expression of a number of genes associated with the SOS response. These results confirm the key role of Dam in the regulation of virulence, and its potential role as a target for the generation of attenuated strains of pathogenic bacteria.